A sample was first applied to the nanoACQUITY UPLC Trapping Column (Waters) utilizing water containing zero.1% formic acid because the cell part.
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Amongst them we recognized histone H3 lysine 27 acetylation (H3K27Ac) as a modification upregulated in CRC; an instance of fragmentation spectrum for H3K27Ac is presented in Additional file 2 : Figure S1.
